ACKERMAN CREEK WATERSHED ASSESSMENT: FISH ASSEMBLAGES OF THREE ILLINOIS RIVER TRIBUTARIES Nerissa N. Michaels, Thad R. Cook and Michael A. McClelland Illinois Natural History Survey, Illinois River Biological Station, 704 N. Schrader Ave., Havana, IL 62644 The Illinois Natural History Survey’s Illinois River Biological Station conducted a baseline assessment of riparian and aquatic biota of three tributaries located in the Ackerman Creek Watershed. This study was conducted in conjunction with the Army Corps of Engineers and the Illinois Department of Natural Resources as part of Illinois River Basin Restoration efforts. Ackerman, Farm and Ten Mile Creeks, located in Peoria and Woodford Counties in central Illinois, were sampled at two 150 meter sites for fish and invertebrate abundance and composition, ancillary water quality and habitat data. Fish sampling gears included a 1500-watt AC electric seine with block nets and a 1600- watt AC backpack shocker. The total number of fish species collected in the three tributaries was 17 and 1 hybrid with a total catch of 2,902 fishes. Although each site exhibited varying degrees of human impact and habitat features, mean catch per site was 483.7 fish and an analysis of variance revealed no significant differences in catch among all six sites (P=0.279). In addition, conflicting results where seen between sites with varying levels of disturbance. Some sites that rendered high levels of disturbance displayed greater species diversity and abundance than other less disturbed sites. Assessment of fish assemblages and composition demonstrated 96.4% of the total catch to be Cyprinids which included sand shiners Notropis stramineus (26.3%), central stonerollers Campostoma anomalum pullum (24.1%), blacknose daces Rhinichthys atratulus (20.5%), river shiners Notropis blennius (14.6%), bluntnose minnows Pimephales notatus (3.4%), creek chubs Semotilus atromaculatus atromaculatus (2.9%) and suckermouth minnows Phenacobius mirabilis (2.8%). These Cyprinids, with the exception to river shiners, also dominated each site individually as they were common to all or most sites. Although there were no statistically significant results, such assessments are of importance because of their allowance of an applicable baseline interpretation of small-scale systems, to guide and identify restoration potentiality and priority, as well as track restoration responses. Keywords: fish assemblage, Illinois River, tributary streams, disturbance