SPATIAL AND TEMPORAL VARIATION IN THE RELATIVE ABUNDANCE OF UPPER MISSISSIPPI RIVER FISH SPECIES Daniel J. Kirby1, and Brian S. Ickes2. 1 Iowa Department of Natural Resources, 206 Rose Street, Bellevue, Iowa, USA 52031 2 U.S. Geological Survey, Biological Resources Division, Upper Midwest Environmental Sciences Center, 575 Lester Avenue, Onalaska, Wisconsin, USA 54650 During 1993-2002, the Long Term Resource Monitoring Program used day electrofishing to make fish collections (N=3,324) within backwater, main channel border, and side channel aquatic areas at randomly selected sites in Pools 4, 8, 13, and 26 of the Upper Mississippi River and the La Grange Pool of the Illinois River. Electrofishing data were used to assess temporal and spatial variation in the relative abundance of fish species (N=50) common to all study areas (i.e., species that were captured in all five study areas during 1993-2002). Specifically this investigation focused on identifying the presence and relative importance of longitudinal-spatial (i.e., among study area), lateral-spatial (i.e., among aquatic area), and temporal (i.e., among year) variation in the proportion of positive electrofishing runs for each species. Among groups variation at the study area, aquatic area, and year factor levels was measured as group sum of squares using a three-factor analysis of variance. Principal components analysis and three-dimensional rotating plots were used to ordinate species based upon factor level and interaction term group sum of squares. These visual representations of longitudinal-spatial, lateral-spatial, and temporal variation in relative abundance can be used by fisheries managers to determine which fish species are most likely to exhibit a relative abundance response to local habitat modifications, climatic variability, or degradation of specific types of aquatic areas. Keywords: fish abundance, Mississippi River, spatial variation, temporal variation, fisheries management