THE EFFECTS OF PURPLE LOOSESTRIFE BIOLOGICAL CONTROL BEETLES (GALERUCELLA SPP.) ON PURPLE LOOSESTRIFE PLOTS ON THE UPPER MISSISSIPPI RIVER NATIONAL WILDLIFE AND FISH REFUGE. Lara Hill U.S. Fish and Wildlife Service, Upper Mississippi River National Wildlife and Fish Refuge, Onalaska, WI 54650. Purple loosestrife (Lythrum salicaria) is an invasive exotic plant in North American wetlands. It displaces native vegetation, forms dense monocultural stands, and eliminates natural food and cover plants essential to many wildlife species. Purple loosestrife is a large aggressive plant with deep roots that produces millions of seeds each year. It spans across 26 states and an estimated 400,000 acres, making control by herbicides, hand pulling, mechanical manipulation, burning, and water manipulations ineffective and infeasible. In 1994, the U.S. Department of Agriculture approved the release of two biological control insects for purple loosestrife. Researchers discovered the insects in the plant's native range of Europe and demonstrated that the insects were safe and effective in reducing purple loosestrife. The leaf and stem-eating beetle Galerucella spp. has been released on six sites of the Upper Mississippi River National Wildlife and Fish Refuge - La Crosse District (Refuge). The root- eating weevil Hylobius tanversoviattauus, has been released on 2 sites. In addition, two Galerucella spp. field insectary sites were created on Trempeleau National Wildlife Refuge. Many factors contribute to the success of the beetles in reducing purple loosestrife. Monitoring has shown a decrease in plant densities and vigor at several of the sites. Keywords: purple loosestrife, biological control, galerucella spp.