USING A TRIAD APPROACH IN ASSESSING HAZARDOUS WASTE SITE LEACHING FROM A SUPERFUND SITE INTO THE WOLF RIVER, A MISSISSIPPI RIVER TRIBUTARY. Christy J. Leppanen1, Pat M. Blanner1, Roger S. Allan1, William H. Benson2 and Kurt J. Maier1 1Department of Biology, The University of Memphis, Memphis, TN 38152 2Department of Pharmacology, University of Mississippi, University, MS 38677 A triad approach was employed in the evaluation of sediment toxicity in the Wolf River, a Mississippi River tributary, adjacent to the North Hollywood Dump, a federally listed superfund site. Chemical analyses were done for 18 organochlorine pesticides, 21 PCB congeners and 10 metals. Sediment toxicity was evaluated using the freshwater invertebrate, Chironomus tentans and Hyallela azteca. Benthic macroinvertebrate diversity and abundance was assessed with a family-level biotic index. Mean Al, Ba, Cd, Cu, Fe, Ni, Pb and Zn concentrations were significantly higher in samples collected in the spring compared to the fall. Both spring and fall sediments exhibited toxicity downstream from, adjacent to, and upstream from the dump. Toxicity was significantly higher in the fall sediment samples, however, a consistent trend was not observed. Toxicity was typically greater in the fall and metal concentrations typically higher in the spring suggesting that metals were not responsible for the observed toxicity. Sediment associated organochlorine pesticide and PCB congener concentrations were all below detectable limits, indicating that there potential contaminants are not contributing to the observed toxicity. No differences were found in benthic macroinvertebrate community structure, which was composed of predominantly pollution-tolerant families, among seasons or river reaches. The communities appear to be limited by the physical characteristics of the river [substrate instability, low TOC (<0.6%), flow]. Sediments in the urban reaches of the Wolf River appear to be degraded; the dump can not be isolated as a source of sediment contamination or toxicity in this study. Keywords: triad assessment, sediment toxicity, hazardous waste site, Wolf River, macroinvertebrate communities Correspondence to: Kurt J. Maier Department of Biology The University of Memphis Memphis, TN 38152 T: (901) 678-2327 F: (901) 678-4746 E: kjmaier@cc.memphis.edu I prefer a platform session